AMPK alpha 2 Antibody (YA4091)

AMPK alpha 2 Antibody (YA4091) is a mouse-derived and non-conjugated IgG1 antibody, targeting to PRKAA2. It can be applicated for WB, IHC-P, ICC/IF, FC, ELISA assays, in the background of human, mouse, rat, monkey, rabbit.

PRKAA2 is the catalytic subunit of AMP-activated protein kinase (AMPK), a central energy sensor kinase that regulates cellular metabolism. Under ATP depletion, AMPK activates catabolic pathways while inhibiting anabolic processes like lipid/protein synthesis and cell proliferation via direct phosphorylation of metabolic enzymes (e.g., ACACA, ACACB, LIPE) or transcriptional regulators (e.g., CRTC2, FOXO3, p53). It promotes lipolysis by phosphorylating CHKalpha2 and enhances glucose uptake via SLC2A4/GLUT4 translocation, potentially through TBC1D4/AS160 phosphorylation (by similar). AMPK modulates insulin signaling by targeting IRS1, PFKFB2/3 (by similar) and regulates chromatin remodeling via H2B phosphorylation (H2BS36ph) under stress (by similar). As a growth controller, it inhibits mTORC1 by phosphorylating RPTOR/TSC2/WDR24 during nutrient scarcity and initiates autophagy through ATG1/ULK1 activation. It also influences circadian rhythms (by similar), Wnt signaling (by similar), and cell polarity. Additional substrates include CASP6 (apoptosis suppression), ACSS2 (nuclear translocation), and MTFR1L (mitochondrial fission).

P54646

Predicted band size: 62 kDa; Observed band size: 62 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human, Mouse, Rat, Monkey, Rabbit

WB: 1/500-1/2000; IHC: 1/100-1/500; IF: 1/50-1/500; FC: 1/200-1/400; ELISA: 1/10000

WB, IHC-P, ICC/IF, FC, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Purified recombinant fragment of human PRKAA2 (AA: 453-552) expressed in E. Coli.