PRAS40 Antibody (YA3179)

PRAS40 Antibody (YA3179) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PRAS40.

AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).

Q96B36

Predicted band size: 27 kDa; Observed band size: 40 kDa

Affinity Purified

Non-conjugated

Unmodified

AB_3105545

Cell Biology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Rat

WB: 1:500-1:1000; IP: 1:20

• 
  Western blot analysis of extracts from Hela (lane 2(20μg), Hela (lane 3(40μg), using PRAS40 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading con-trol antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB, IP

Liquid

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthetic peptide of human PRAS40