PMS2 Antibody (YA4088)

PMS2 Antibody (YA4088) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to PMS2.

PMS2 is a component of the post-replicative DNA mismatch repair system (MMR). It heterodimerizes with MLH1 to form MutL alpha, which participates in the initiation of DNA repair. MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) first binds to the dsDNA mismatch, followed by recruitment of MutL alpha to the heteroduplex. In the presence of RFC and PCNA, assembly of the MutL-MutS-heteroduplex ternary complex is sufficient to activate the endonuclease activity of PMS2, introducing single-strand breaks near the mismatch to create new entry points for exonuclease EXO1 to degrade the mismatch-containing strand. DNA methylation prevents cleavage, ensuring that only the newly mutated DNA strand is corrected. MutL alpha (MLH1-PMS2) also physically interacts with the clamp loader subunits of DNA polymerase III, suggesting a role in recruiting DNA polymerase III to the MMR site. Additionally, PMS2 is implicated in DNA damage signaling, a process that induces cell cycle arrest and can lead to apoptosis in cases of severe DNA damage. PMS2 possesses ATPase activity, but ATP hydrolysis may not be necessary for proficient mismatch repair in the absence of gross structural changes.

P54278

Predicted band size: 96 kDa; Observed band size: 96 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal Antibody

Mouse

Human

WB: 1/500-1/2000; IF: 1/500-1/2000; FC: 1/200-1/400; ELISA: 1/10000

WB, ICC/IF, FC, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG2b

Purified recombinant fragment of human PMS2 (AA: 748-851) expressed in E. Coli.