PINK1 Antibody (YA4465)

PINK1 Antibody (YA4465) is a mouse-derived and non-conjugated IgG1 antibody, targeting to PINK1. It can be applicated for IHC-P, FC, ELISA assays, in the background of human.

PINK1 Protein operates as a serine/threonine-protein kinase with a substrate preference for proline and a predilection for basic residues such as arginine. Vital for mitochondrial homeostasis, it orchestrates pathways that sustain mitochondrial health and function. During cellular stress, PINK1 safeguards against mitochondrial dysfunction by phosphorylating proteins like park and Drp1, coordinating quality control mechanisms for damaged mitochondria. Depending on the severity of mitochondrial damage, PINK1's activity ranges from preventing apoptosis and stimulating mitochondrial biogenesis to regulating dynamics and eliminating severely damaged mitochondria through mitophagy. Particularly crucial for cells under stress or altered metabolic environments, such as spermatids, muscle cells, and dopaminergic neurons, PINK1 mediates the translocation and activation of park at the outer mitochondrial membrane (OMM). This phosphorylation-dependent recruitment of park to the OMM facilitates mitochondrial clearance and regulates fission/fusion dynamics. Additionally, PINK1 is involved in mitochondrial regeneration processes, promoting biogenesis, initiating repair mechanisms, and influencing metabolic pathways independent of its role in ATP production.

Q9BXM7

Predicted band size: 63 kDa; Observed band size: 63 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human

IHC: 1/200-1/1000; FC: 1/200-1/400; ELISA: 1/10000

IHC-P, FC, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Purified recombinant fragment of human PINK1 (AA: 112-273) expressed in E. Coli.