Phospho-β Catenin(Ser675) Antibody (YA5779)

Phospho-β Catenin(Ser675) Antibody (YA5779) is a rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to phosphorylated β Catenin (Ser675). It can be applicated for WB, ICC/IF, IP, ELISA assays, in the background of human, mouse, rat.

Beta-catenin, a pivotal player in the canonical Wnt signaling pathway, dynamically regulates cellular processes in response to Wnt ligands. In the absence of Wnt, beta-catenin participates in a complex with AXIN1, AXIN2, APC, CSNK1A1, and GSK3B, promoting its phosphorylation and ubiquitination, leading to subsequent degradation by the proteasome. Upon Wnt activation, beta-catenin accumulates in the nucleus, acting as a coactivator for transcription factors of the TCF/LEF family, thereby activating Wnt-responsive genes. This multifaceted protein also plays a role in cell adhesion, acting as a component of an E-cadherin:catenin adhesion complex. Beyond these functions, beta-catenin emerges as a negative regulator of centrosome cohesion and exhibits roles in insulin internalization, inhibition of anoikis, and promotion of neurogenesis. It modulates chondrocyte differentiation, positively regulates odontoblast differentiation during tooth germ formation, and interacts with various proteins, including regulators and effectors of Wnt signaling, highlighting its central role in diverse cellular processes. Beta-catenin's complex network of interactions underscores its significance in orchestrating cellular responses to intricate signaling cues, contributing to the regulation of critical cellular functions.

P35222

Predicted band size: 85 kDa; Observed band size: 85 kDa

Protein A

Non-conjugated

Phosphorylated

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:2000; ICC/IF: 1:200-1:1000; ELISA: 1:5000-1:20000; IP: 1:50-1:200;

WB, ICC/IF, IP, ELISA

Liquid

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG