Phospho-YAP1 (Ser127) Antibody (YA137)

Cat. No.: HY-P80481: Data Sheet COA
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Phospho-YAP1 (Ser127) Antibody (YA137) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-YAP1 (Ser127).

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Phospho-YAP1 (Ser127) Antibody (YA137) Featured Recommendations:

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

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Description	Phospho-YAP1 (Ser127) Antibody (YA137) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-YAP1 (Ser127).
Background	YAP1, a multifaceted transcriptional regulator, serves as a critical downstream target in the Hippo signaling pathway, orchestrating organ size control and tumor suppression by modulating proliferation and apoptosis. The pathway involves a kinase cascade where STK3/MST2 and STK4/MST1, in coordination with SAV1, phosphorylate and activate LATS1/2, leading to the phosphorylation and inactivation of YAP1 and WWTR1/TAZ oncoproteins. YAP1 is pivotal in regulating tissue tension and 3D tissue shape by influencing cortical actomyosin network formation through ARHGAP18. Moreover, it plays a key role in controlling cell proliferation, with its nuclear translocation inhibited by LATS1/2 phosphorylation. YAP1's collaboration with TEAD transcription factors is essential for stimulating gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Additionally, it suppresses ciliogenesis by acting as a transcriptional corepressor of TEAD4 target genes AURKA and PLK1, and in conjunction with WWTR1, it regulates TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation. YAP1 further activates the C-terminal fragment (CTF) of ERBB4 isoform 3.
Tag	Free
Gene ID	10413 [NCBI]
SwissProt ID	P46937
Molecular Weight	Predicted band size: 54 kDa; Observed band size: 70 kDa
Purity	Protein A affinity purified.
Subcellular Location	Cytoplasm, Nucleus.
Conjugation	Non-conjugated
Modification	Phosphorylated
RRID	AB_3102554
Research Field	Signal Transduction
Product Categories	Primary Antibody; Recombinant Rabbit Monoclonal Antibody
Clonality	Recombinant, Monoclonal
Host	Rabbit
Reactivity	Human, Mouse
Dilution Ratio	WB: 1:500-1:1000; IHC-P: 1:50-1:200
Application	WB, IHC-P
Appearance	Liquid
Formulation	Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.
Storage & Stability	Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping	Shipping with blue ice.
Isotype	IgG
Sensitivity	Endogenous
Immunogen	Synthetic phosphopeptide corresponding to residues surrounding Ser127 of Human YAP1.The exact sequence is proprietary to MCE.
Database	Entrez Gene: 10413 Human ; 22601 Mouse ; SwissProt: P46937 Human ; P46938 Mouse ; OMIM: 120433 Human
Documentation	Select Batch: Data Sheet (235 KB) SDS (251 KB) COA (206 KB) User Guide for Antibodies (1077 KB)

Phospho-YAP1 (Ser127) Antibody (YA137) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Phospho-YAP1 (Ser127) Antibody (YA137)

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