Phospho-ULK1(Ser757) Antibody (YA5857)

Phospho-ULK1(Ser757) Antibody (YA5857) is a rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to phosphorylated ULK1 (Ser757). It can be applicated for WB, ICC/IF, IP, ELISA assays, in the background of human, mouse, rat.

ULK1 is a serine/threonine-protein kinase primarily involved in autophagy in response to starvation. It plays a crucial role in the formation of autophagophores, the precursors of autophagosomes, acting upstream of phosphatidylinositol 3-kinase PIK3C3. ULK1 is part of regulatory feedback loops in autophagy, functioning both as a downstream effector and negative regulator of mTORC1 via interaction with RPTOR. It is activated by AMPK-mediated phosphorylation and, in turn, regulates AMPK activity by phosphorylating its subunits PRKAA1, PRKAB2, and PRKAG1. ULK1 may also phosphorylate ATG13/KIAA0652 and RPTOR, though further evidence is needed. Additionally, ULK1 is essential for early neuronal differentiation and granule cell axon formation. It regulates autophagy by phosphorylating SESN2, SQSTM1, FLCN, and AMBRA1. Phosphorylation of AMBRA1 releases it from cytoskeletal docking sites to promote autophagosome nucleation. ULK1 also inhibits autophagy by phosphorylating ATG4B, reducing its proteolytic activation and delipidation activities.

O75385

Predicted band size: 113 kDa; Observed band size: 150 kDa

Protein A

Non-conjugated

Phosphorylated

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Monoclonal

Rabbit

Human, Mouse, Rat

Mouse

WB: 1:2000-1:10000; ICC/IF: 1:200-1:1000; ELISA: 1:5000-1:20000; IP: 1:50-1:200;

WB, ICC/IF, IP, ELISA

WB

Liquid

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG