Phospho-STAT3 (Tyr705) Antibody (YA146)

Phospho-STAT3 (Tyr705) Antibody (YA146) is a non-conjugated and Rabbit origined monoclonal antibody about 88 kDa, targeting to Phospho-STAT3(Tyr705). It can be used for WB,IHC-P,IP assays with tag free, in the background of Human, Mouse.

Free

P40763

Predicted band size: 88 kDa

Protein A affinity purified.

Cytoplasm, Nucleus.

Non-conjugated

Phosphorylated

AB_3102403

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse

WB: 1:500-1:2,000 ;IHC-P: 1:50-1:200 ;IP: Use at an assay dependent concentration.

• 
  Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using Phospho-STAT3 (Tyr705) Antibody (YA146). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80282, 1/500) in4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using Phospho-STAT3 (Tyr705) Antibody (YA146). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80282, 1/500) in4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P, IP

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic phosphopeptide corresponding to residues surrounding Tyr705 of Human STAT3.The exact sequence is proprietary to MCE.