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  5. Phospho-PKC (Ser660) Antibody (YA1394)

Phospho-PKC (Ser660) Antibody (YA1394)

Cat. No.: HY-P81649
User Guide for Antibodies Technical Support

Phospho-PKC (Ser660) Antibody (YA1394) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA1394), targeting Phospho-PKC (Ser660), with a predicted molecular weight of 77 kDa (observed band size: 78-85 kDa). Phospho-PKC (Ser660) Antibody (YA1394) can be used for WB, IHC-P experiment in human, rat background.

For research use only. We do not sell to patients.

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10 μL Get quote 3 - 4 weeks 2 - 3 weeks 2 - 3 weeks
50 μL Get quote 3 - 4 weeks 2 - 3 weeks 2 - 3 weeks
100 μL Get quote 3 - 4 weeks 2 - 3 weeks 2 - 3 weeks
250 μL   Get quote  
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Top Publications Citing Use of Products

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

Phospho-PKC (Ser660) Antibody (YA1394) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA1394), targeting Phospho-PKC (Ser660), with a predicted molecular weight of 77 kDa (observed band size: 78-85 kDa). Phospho-PKC (Ser660) Antibody (YA1394) can be used for WB, IHC-P experiment in human, rat background.
Background

PKC alpha is a calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that regulates cell proliferation, apoptosis, differentiation, migration, adhesion, tumorigenesis, cardiac hypertrophy, angiogenesis, platelet function, and inflammation by directly phosphorylating targets like RAF1, BCL2, CSPG4, and TNNT2/CTNT or activating signaling cascades involving MAPK1/3 (ERK1/2) and RAP1GAP. It promotes cell growth in glioma cells via RAF1 phosphorylation or CDKN1A upregulation, while inducing cell cycle arrest in PMA-stimulated intestinal cells. During macrophage differentiation, it translocates to the nucleus and modulates desmosomal adhesion post-wounding. PKC alpha enhances cell motility by phosphorylating CSPG4 and is overexpressed in cancers, acting as an oncogene. It negatively regulates myocardial contractility but positively controls angiogenesis and platelet aggregation. The kinase phosphorylates cardiac troponin T (TNNT2/CTNT) to reduce myocardial contractility and stabilizes VEGFA mRNA to promote angiogenesis. In platelets, it mediates granule release via CD36/GP4 and activates ITGA2B-ITGB3 integrin through RAP1GAP. PKC alpha also modulates LPS-induced macrophage functions and negatively regulates NF-κB via IL1A-dependent NFKBIA induction. It phosphorylates EIF4G1, KIT, and ATF2 to influence translation and transcription. Similar functions (By similarity) include SOCS2 phosphorylation for degradation and KLHL3 phosphorylation to disrupt WNK4 binding. Recent studies (PubMed:36040231) show it activates LRRK1 to regulate RAB proteins in intracellular trafficking.
Gene ID
SwissProt ID
Synonyms
PRKCA; PKCA; PRKACA; Protein kinase C alpha type; PKC-A; PKC-alpha
Molecular Weight

Predicted band size: 77 kDa; Observed band size: 78-85 kDa
Purity

Affinity Purified
Conjugation

Non-conjugated
Modification

Phosphorylated
Research Field

Signal Transduction
Product Categories

Primary Antibody; Recombinant Rabbit Monoclonal Antibody
Clonality

Recombinant, Monoclonal
Host

Rabbit
Reactivity

Human, Rat
Dilution Ratio

WB: 1/500-1/1000;IHC: 1/50-1/100
Application

WB, IHC-P
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Isotype

IgG
Sensitivity

Endogenous
Immunogen

A synthetic phosphopeptide corresponding to residues surrounding Ser660 of human PKC
Database

Entrez Gene: 5578 Human ; 5579 Human ; 5580 Human ; 5581 Human ; 5582 Human ; 5590 Human ; 18750 Mouse ; 18751 Mouse ; 18752 Mouse ; 18753 Mouse ; 18754 Mouse ; 18762 Mouse ; 170538 Rat 24680

SwissProt: P05129 Human ; P05771 Human ; P17252 Human ; Q02156 Human ; Q05513 Human ; Q05655 Human ; P20444 Mouse ; P23298 Mouse ; P63318 Mouse ; P68404 Mouse ; Q02956 Mouse ; P05696 Rat P09217

OMIM: 605361 Human

Documentation
User Guide for Antibodies (1077 KB)

Phospho-PKC (Ser660) Antibody (YA1394) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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