Phospho-PERK (Thr980) Antibody

Cat. No.: HY-P81190: Data Sheet COA
SDS
User Guide for Antibodies
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Phospho-PERK (Thr980) Antibody is an unconjugated, approximately 119 kDa, rabbit-derived, anti-PERK (Thr980) polyclonal antibody. Phospho-PERK (Thr980) Antibody can be used for: WB, ELISA, IHC-P, IHC-F, Flow-Cyt, IF expriments in human, mouse, rat, and predicted: dog, pig, cow, rabbit background without labeling.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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•SSRN. 2025 Mar 13.

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

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Description	Phospho-PERK (Thr980) Antibody is an unconjugated, approximately 119 kDa, rabbit-derived, anti-PERK (Thr980) polyclonal antibody. Phospho-PERK (Thr980) Antibody can be used for: WB, ELISA, IHC-P, IHC-F, Flow-Cyt, IF expriments in human, mouse, rat, and predicted: dog, pig, cow, rabbit background without labeling.
Background	PERK is a metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to various stresses, such as the unfolded protein response (UPR). As a key effector of the integrated stress response (ISR) to unfolded proteins, PERK specifically recognizes and binds misfolded proteins, leading to its activation and phosphorylation of EIF2S1/eIF-2-alpha. This phosphorylation converts EIF2S1/eIF-2-alpha into a global protein synthesis inhibitor, attenuating cap-dependent translation while initiating preferential translation of ISR-specific mRNAs, such as the transcriptional activators ATF4 and QRICH1, enabling ATF4- and QRICH1-mediated reprogramming. The PERK-mediated unfolded protein response enhances mitochondrial oxidative phosphorylation by promoting ATF4-dependent expression of COX7A2L/SCAF1, thereby increasing respiratory chain supercomplex formation. Unlike most subcellular compartments, mitochondria are protected from PERK-mediated UPR due to inhibition by ATAD3A at mitochondria-endoplasmic reticulum contact sites (by similar). Additionally, PERK phosphorylates NFE2L2/NRF2 under stress, promoting its release from the BCR(KEAP1) complex, leading to nuclear accumulation and activation of NFE2L2/NRF2. PERK serves as a critical effector of UPR-induced G1 growth arrest caused by cyclin-D1 (CCND1) loss and is involved in regulating mitochondrial morphology and function.
Gene ID	9451 [NCBI]
SwissProt ID	Q9Z2B5
Synonyms	p-PERK(Thr980); PERK(Phospho Thr980); PERK(Phospho T980); mo(Thr980)/hu (Thr982)/rat (Thr974); HRI; HsPEK; Pancreatic eIF2-alpha kinase; PEK; PRKR like endoplasmic reticulum kinase; WRS; DKFZp781H1925; EC 2.7.11.1; EIF2AK3; Eukaryotic translation initiation factor 2 alpha kinase 3; Heme regulated EIF2 alpha kinase.
Molecular Weight	Predicted band size: 119 kDa; Observed band size: 120 kDa
Purity	affinity purified
Subcellular Location	Endoplasmic reticulum membrane; Single-pass type I membrane protein.
Conjugation	Non-conjugated
Modification	Phosphorylated
RRID	AB_3103093
Product Categories	Primary Antibody; Rabbit Polyclonal Antibody
Clonality	Polyclonal
Host	Rabbit
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Pig, Cow, Rabbit Note: The predicted reactivity is for reference only, not as a product performance guarantee.
Dilution Ratio	WB: 1:500-2000;ELISA: 1:5000-10000;IHC-P: 1:100-500;IHC-F: 1:100-500;Flow-Cyt: 2ug/Test;IF: 1:100-500
Application	WB, ELISA, IHC-P, IHC-F, FC, ICC/IF
Appearance	Liquid
Formulation	Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage & Stability	Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping	Shipping with blue ice.
Isotype	IgG
Sensitivity	Endogenous
Immunogen	KLH conjugated synthesised phosphopeptide derived from mouse PERK around the phosphorylation site of Thr980: H(p-T)GQ
Database	Entrez Gene: 9451 Human ; 13666 Mouse ; 29702 Rat SwissProt: Q9NZJ5 Human ; Q9Z2B5 Mouse ; Q9Z1Z1 Rat OMIM: 226980 Human
Documentation	Select Batch: Data Sheet (235 KB) SDS (252 KB) COA (191 KB) User Guide for Antibodies (1077 KB)

Phospho-PERK (Thr980) Antibody Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Phospho-PERK (Thr980) Antibody

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