Phospho-PDPK1(Ser241) Antibody (YA5781)

Phospho-PDPK1(Ser241) Antibody (YA5781) is a rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to phosphorylated PDPK1 (Ser241). It can be applicated for WB, ICC/IF, IP, ELISA assays, in the background of human, mouse, rat.

PDPK1, a serine/threonine kinase, assumes a central role as a master kinase within the AGC family, orchestrating the phosphorylation and activation of various downstream protein kinases. Its diverse targets include protein kinase B (PKB/AKT1, PKB/AKT2, PKB/AKT3), p70 ribosomal protein S6 kinase (RPS6KB1), p90 ribosomal protein S6 kinase (RPS6KA1, RPS6KA2, and RPS6KA3), cyclic AMP-dependent protein kinase (PRKACA), protein kinase C (PRKCD and PRKCZ), serum and glucocorticoid-inducible kinase (SGK1, SGK2, and SGK3), p21-activated kinase-1 (PAK1), and protein kinase PKN (PKN1 and PKN2). PDPK1 plays a pivotal role in transducing insulin signals by activating PKB/AKT1, thereby regulating downstream targets involved in cell proliferation, survival, glucose and amino acid uptake, and storage. Additionally, it exerts regulatory functions, negatively modulating TGF-beta-induced signaling and activating PPARG transcriptional activity to promote adipocyte differentiation. PDPK1 further participates in diverse cellular processes, including the activation of the NF-kappa-B pathway via IKKB phosphorylation, regulation of focal adhesions by angiotensin II, control of pancreatic cell proliferation, and modulation of Ca(2+) entry and Ca(2+)-activated K(+) channels in mast cells. It also plays critical roles in endothelial cell motility, cardiac homeostasis, thymocyte development, and provides negative feedback inhibition in toll-like receptor-mediated NF-kappa-B activation in macrophages, with isoform 3 being catalytically inactive.

O15530

Predicted band size: 63 kDa; Observed band size: 63 kDa

Protein A

Non-conjugated

Phosphorylated

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:2000-1:10000; ICC/IF: 1:200-1:1000; ELISA: 1:5000-1:20000; IP: 1:50-1:200;

WB, ICC/IF, IP, ELISA

Liquid

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG