Phospho-NF-KB p105/p50(Ser337) Antibody

Cat. No.: HY-P81049: Data Sheet COA
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Phospho-NF-KB p105/p50(Ser337) Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Phospho-NF-KB p105/p50(Ser337).

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Phospho-NF-KB p105/p50(Ser337) Antibody Featured Recommendations:

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

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Description	Phospho-NF-KB p105/p50(Ser337) Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Phospho-NF-KB p105/p50(Ser337).
Background	NFKB1 protein serves as a pivotal component of the pleiotropic transcription factor NF-kappa-B, found ubiquitously across various cell types. This transcription factor acts as a central mediator in signal transduction pathways associated with diverse biological processes, including inflammation, immunity, differentiation, cell growth, tumorigenesis, and apoptosis. NFKB1 participates in homo- or heterodimeric complexes, with the p65-p50 heterodimer being the most prevalent. These dimers selectively bind to kappa-B sites in target gene DNA, exhibiting distinct preferences and affinities. NF-kappa-B regulation involves intricate post-translational modifications, subcellular compartmentalization, and interactions with cofactors or corepressors. In an inactive state, NF-kappa-B complexes associate with NF-kappa-B inhibitor (I-kappa-B) family members in the cytoplasm. Activation ensues through phosphorylation and degradation of I-kappa-B, liberating active NF-kappa-B complexes for nuclear translocation. NFKB1 exhibits dual functions, contributing to cytoplasmic retention of NF-kappa-B proteins and generating the active p50 subunit through cotranslational processing. This processing, mediated by the proteasome, ensures the independent functionality of p50 and p105. The p50 subunit binds to kappa-B consensus sequences in the enhancer regions of genes involved in immune response and acute phase reactions. Additionally, NFKB1, in association with MAP3K8, represses MAP3K8-induced MAPK signaling, with active MAP3K8 released through proteasome-dependent degradation of NFKB1/p105.
Gene ID	4790 [NCBI]
SwissProt ID	P19838
Synonyms	NFKB1; NF-KB; Nuclear factor NF-kappa-B p105 subunit; DNA-binding factor KBF1; EBP-1; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1; DNA-binding factor KBF1; EBP-1; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1; Nuclear factor NF-kappa-B p50 subunit; NFkB p105 / p50; NFkB p105/p50; nuclear factor kappa B subunit 1; NF-kB; CVID12; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B1; NF-kappabeta;
Molecular Weight	Predicted band size: 48/105 kDa; Observed band size: 48/105 kDa
Purity	Affinity Purified
Subcellular Location	Nucleus. Cytoplasm.
Conjugation	Non-conjugated
Modification	Phosphorylated
RRID	AB_3103041
Research Field	Cell Biology
Product Categories	Primary antibody；Rabbit Polyclonal Antibody
Clonality	Polyclonal
Host	Rabbit
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Pig, Cow Note: The predicted reactivity is for reference only, not as a product performance guarantee.
Dilution Ratio	WB: 1:500-2000; IHC-P: 1:100-500; IHC-F: 1:100-500; FC: 1μg/Test; IF: 1:100-500; ELISA: 1:5000-10000;
Application	WB, IHC-P, IHC-F, FC, ICC/IF, ELISA
Appearance	Liquid
Formulation	Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage & Stability	Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping	Shipping with blue ice.
Isotype	IgG
Sensitivity	Endogenous
Immunogen	KLH conjugated Synthesised phosphopeptide derived from human NF KappaB p105 around the phosphorylation site of Ser337: RK(p-S)DL
Database	Entrez Gene: 4790 Human ; 18033 Mouse ; 81736 Rat SwissProt: P19838 Human ; P25799 Mouse ; Q63369 Rat OMIM: 616576 Human
Documentation	Select Batch: Data Sheet (235 KB) SDS (252 KB) COA (206 KB) User Guide for Antibodies (1077 KB)

Phospho-NF-KB p105/p50(Ser337) Antibody Related Classifications

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Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Phospho-NF-KB p105/p50(Ser337) Antibody

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