Phospho-MLKL(S358) Antibody (YA5761)

Phospho-MLKL(S358) Antibody (YA5761) is a mouse-derived and non-conjugated monoclonal antibody, targeting to MLKL (S358). It can be applicated for ICC/IF, IHC-P assays, in the background of human.

MLKL, a pseudokinase, plays a pivotal role in TNF-induced necroptosis, a programmed cell death process characterized by plasma membrane damage and calcium influx. Despite lacking intrinsic protein kinase activity, MLKL is activated upon phosphorylation by RIPK3. This activation leads to homotrimerization, plasma membrane localization, and execution of necroptosis. Additionally, in response to orthomyxoviruses infection, MLKL can undergo nuclear necroptosis triggered by ZBP1 activation, resulting in disruption of the nuclear envelope and release of cellular DNA into the cytosol. MLKL's necroptotic function is facilitated by its binding to highly phosphorylated inositol phosphates, such as inositolhexakisphosphate (InsP6). This binding not only mediates the release of an N-terminal auto-inhibitory region but also requires RIPK3-dependent phosphorylation for full activation. Necrosulfonamide, a specific inhibitor of necroptosis, targets Cys-86 and effectively inhibits MLKL function.

Q8NB16

Observed band size: 54 kDa

affinity chromatography.

Non-conjugated

Phosphorylated

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human

ICC/IF: 1:50-200; IHC: 1:100-200

ICC/IF, IHC-P

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

Synthetic Peptide of phospho-MLKL (S358)