Phospho-MLKL (Ser345) Antibody (YA174)

Phospho-MLKL (Ser345) Antibody (YA174) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-MLKL (Ser345).

MLKL, a pseudokinase, plays a pivotal role in TNF-induced necroptosis, a programmed cell death process characterized by plasma membrane damage and calcium influx. Despite lacking intrinsic protein kinase activity, MLKL is activated upon phosphorylation by RIPK3. This activation leads to homotrimerization, plasma membrane localization, and execution of necroptosis. Additionally, in response to orthomyxoviruses infection, MLKL can undergo nuclear necroptosis triggered by ZBP1 activation, resulting in disruption of the nuclear envelope and release of cellular DNA into the cytosol. MLKL's necroptotic function is facilitated by its binding to highly phosphorylated inositol phosphates, such as inositolhexakisphosphate (InsP6). This binding not only mediates the release of an N-terminal auto-inhibitory region but also requires RIPK3-dependent phosphorylation for full activation. Necrosulfonamide, a specific inhibitor of necroptosis, targets Cys-86 and effectively inhibits MLKL function.

Free

Q9D2Y4 Mouse

Predicted band size: 54 kDa; Observed band size: 54 kDa

Protein A affinity purified.

Cell membrane, Cytoplasm, Nucleus.

Non-conjugated

Phosphorylated

AB_3644175

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Mouse

WB: 1:500; IHC-P: 1:50-1:100

WB, IHC-P

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic phosphopeptide corresponding to residues surrounding Ser345 of Mouse MLKL.AA range:314-363.