Phospho-JNK (Thr183) Antibody

Phospho-JNK (Thr183) Antibody is a non-conjugated and Rabbit origined polyclonal antibody about 48 kDa, targeting to Phospho-JNK (Thr183). It can be used for WB,IHC-P,ICC/IF,IP,FC assays with tag free, in the background of Human, Mouse, Rat.

JNK1, a serine/threonine-protein kinase, intricately regulates diverse cellular processes, including cell proliferation, differentiation, migration, transformation, and programmed cell death. Activation of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway, stimulated by extracellular factors such as pro-inflammatory cytokines or physical stress, involves the phosphorylation and activation of JNK1 by the dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7. In the ensuing cascade, JNK1 phosphorylates key transcription factors, particularly components of AP-1 such as JUN, JDP2, and ATF2, thereby modulating AP-1 transcriptional activity. JNK1 also exerts regulatory control over replication initiation by phosphorylating the replication licensing factor CDT1, disrupting its interaction with the histone H4 acetylase HBO1. This event impedes the acetylation required for replication origins. Additionally, JNK1 plays a pivotal role in apoptosis induction by phosphorylating critical regulatory factors, including p53/TP53 and Yes-associates protein YAP1. Its involvement in diverse cellular pathways encompasses contributions to T-cell differentiation, survival of erythroid cells, autophagy activation, and regulation of microtubule dynamics in neurons. JNK1's extensive substrate repertoire includes heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, the E3 ligase ITCH, the circadian clock regulator CLOCK-BMAL1, and the heat shock transcription factor HSF1, among others. Notably, JNK1 isoforms exhibit distinct binding patterns, with beta-1 preferentially binding to c-Jun, while alpha-1, alpha-2, and beta-2 display a similar low level of binding to both c-Jun and ATF2, although phosphorylation efficiency remains consistent across isoforms.

Free

P45983 / P45984 / P53779

Predicted band size: 48 kDa; Observed band size: 46/54 kDa

affinity purified

Non-conjugated

Phosphorylated

AB_3102216

Signal Transduction

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:1000 ;IHC: 1:50-1:100 ;IF: 1:50-1:200 ;IP: 1:20 ;FC: 1:50-1:100

• 
  Western blot analysis was performed on extracts from 293T+CA (lane 1, 20 μg), and 3T3+CA (lane 2, 20 μg) using Phospho-JNK (Thr183) Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P2804, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

WB, IHC-P, ICC/IF, IP, FC

Liquid

Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic phosphopeptide corresponding to residues surrounding Thr183 of Human Phospho-JNK1/2/3.The exact sequence is proprietary to MCE.