Phospho-HER2(Tyr1221/Tyr1222) Antibody

Phospho-HER2(Tyr1221/Tyr1222) Antibody is a rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to phosphorylated HER2 (Tyr1221/Tyr1222). It can be applicated for WB, IHC-P, IP, ELISA assays, in the background of human, mouse.

ERBB2 is a protein tyrosine kinase that forms part of several cell surface receptor complexes but typically requires a coreceptor for ligand binding. It is an essential component of the neuregulin-receptor complex, though neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. ERBB2 regulates the outgrowth and stabilization of peripheral microtubules (MTs). Upon activation, the MEMO1-RHOA-DIAPH1 signaling pathway induces the phosphorylation and inhibition of GSK3B at the cell membrane, preventing APC and CLASP2 phosphorylation and enabling their membrane association. Subsequently, membrane-bound APC facilitates the localization of MACF1 to the cell membrane, which is critical for microtubule capture and stabilization. In the nucleus, ERBB2 is involved in transcriptional regulation, binding to the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter to activate transcription. It also contributes to CDKN1A transcriptional activation via STAT3 and SRC and enhances rRNA gene transcription by RNA polymerase I, promoting protein synthesis and cell growth.

P04626

Predicted band size: 138 kDa；Observed band size: 180 kDa

Affinity Chromatography

Non-conjugated

Phosphorylated

Cancer

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Human, Mouse

WB: 1/500-1/1000; IHC: 1/50-1/100; IP: 1/20; ELISA: 1/10000

WB, IHC-P, IP, ELISA

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

The antiserum was produced against synthesized peptide derived from human HER2 around the phosphorylation site of Tyr1221/Tyr1222.