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  4. Phospho-GSK3 (Tyr216/Tyr279) Antibody (YA194)

Phospho-GSK3 (Tyr216/Tyr279) Antibody (YA194)

Cat. No.: HY-P80819
COA User Guide for Antibodies Technical Support

Phospho-GSK3 (Tyr216/Tyr279) Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 51 kDa, targeting to Phospho-GSK3 (Tyr216/Tyr279). It can be used for WB,IP assays with tag free, in the background of Mouse, Rat.

For research use only. We do not sell to patients.

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10 μL In-stock
50 μL In-stock
100 μL In-stock
250 μL   Get quote  

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Top Publications Citing Use of Products

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

Phospho-GSK3 (Tyr216/Tyr279) Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 51 kDa, targeting to Phospho-GSK3 (Tyr216/Tyr279). It can be used for WB,IP assays with tag free, in the background of Mouse, Rat.

Background

GSK3α protein, a constitutively active kinase, plays a crucial role as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling, and transcription factor and microtubule regulation. It exerts its regulatory function by phosphorylating and inactivating key targets such as glycogen synthase (GYS1 or GYS2), CTNNB1/beta-catenin, APC, and AXIN1. Primed phosphorylation is a requisite for the majority of its substrates. GSK3α contributes to insulin's control of glycogen synthesis by inhibiting GYS1 activity and thus glycogen synthesis, particularly in the liver. Additionally, it is implicated in the regulation of insulin resistance and plays a role in Wnt signaling by modulating the level and transcriptional activity of nuclear CTNNB1/beta-catenin. GSK3α also participates in amyloid precursor protein (APP) processing associated with Alzheimer's disease, regulates replication in pancreatic beta-cells, and is crucial for the establishment of neuronal polarity and axon outgrowth. Furthermore, it controls cell apoptosis in response to growth factor deprivation, negatively regulates the extrinsic apoptotic signaling pathway, and promotes the formation of an anti-apoptotic complex at death receptors. GSK3α also acts as a regulator of autophagy by mediating phosphorylation of KAT5/TIP60 under starvation conditions, promoting acetylation of key autophagy regulators. Additionally, it negatively regulates the mTORC2 complex by phosphorylating RICTOR, leading to its ubiquitination and degradation.

Tag

Free

Gene ID
SwissProt ID
Synonyms
Serine/threonine-protein kinase GSK3A; Serine/threonine-protein kinase GSK3B
Molecular Weight

Predicted band size: 51 kDa; Observed band size: 47-51 kDa

Purity

affinity purified

Conjugation

Non-conjugated

Modification

Phosphorylated

RRID
Research Field

Neuroscience

Product Categories

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Clonality

Recombinant, Monoclonal

Host

Rabbit

Reactivity

Mouse, Rat

Dilution Ratio

WB: 1:500-1:1,000 ;IP: 1:20

Application

WB, IP

Appearance

Liquid

Formulation

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Isotype

IgG

Sensitivity

Endogenous

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Tyr216 of Human GSK3 alpha.The exact sequence is proprietary to MCE.

Database
Documentation

Phospho-GSK3 (Tyr216/Tyr279) Antibody (YA194) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Phospho-GSK3 (Tyr216/Tyr279) Antibody (YA194)
Cat. No.:
HY-P80819
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