Phospho-FOXO3A(Ser253) Antibody (YA6240)

Phospho-FOXO3A(Ser253) Antibody (YA6240) is a rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to phosphorylated FOXO3A (Ser253). It can be applicated for WB, IHC-P, ICC/IF, IP, ELISA assays, in the background of human, mouse, rat.

FOXO3a is a transcriptional activator that recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3', regulating processes such as apoptosis and autophagy. In skeletal muscle, it acts as a positive regulator of autophagy: under starvation conditions, FOXO3a enters the nucleus after dephosphorylation and binds to the promoters of autophagy-related genes (e.g., GABARAP1L, MAP1LC3B, and ATG12), activating their expression and leading to proteolysis of muscle proteins (by similar). It triggers apoptosis in the absence of survival factors, including neuronal cell death under oxidative stress. Additionally, upon phosphorylation by MAPKAPK5, FOXO3a promotes the expression of miR-34b and miR-34c, post-transcriptional regulators of MYC. During metabolic stress, it translocates to mitochondria to enhance mtDNA transcription. FOXO3a also regulates chondrogenic commitment of skeletal progenitor cells by activating SOX9 expression under low lipid conditions (by similar) and suppresses fatty acid oxidation. Moreover, it plays a key role in regulatory T-cell (Treg) differentiation by activating FOXP3 expression.

O43524

Predicted band size: 71 kDa; Observed band size: 97 kDa

Protein A

Non-conjugated

Phosphorylated

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Monoclonal

Rabbit

Human, Mouse, Rat

IHC: 1:200-1:1000; WB: 1:1000-1:5000; ICC/IF: 1:200-1:1000; ELISA: 1:5000-1:20000; IP: 1:50-1:200,

WB, IHC-P, ICC/IF, IP, ELISA

Liquid

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG