Phospho-DRP1(Ser616) Antibody (YA5812)

Phospho-DRP1(Ser616) Antibody (YA5812) is a rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to phosphorylated DRP1 (Ser616). It can be applicated for WB, ICC/IF, IP, ELISA assays, in the background of human, mouse, rat.

DNM1L is a key protein involved in mitochondrial and peroxisomal division. It mediates membrane fission by oligomerizing into membrane-associated tubular structures that constrict and sever mitochondrial membranes through a GTP hydrolysis-dependent mechanism (PubMed:23530241, PubMed:23584531, PubMed:33850055). Recruitment to scission sites is facilitated by membrane receptors such as MFF, MIEF1, and MIEF2 (PubMed:23283981, PubMed:23921378, PubMed:29899447). GTP hydrolysis triggers dissociation from receptors, allowing DNM1L filaments to form closed rings likely sufficient for membrane scission (PubMed:29899447). It acts downstream of PINK1 to promote mitochondrial fission in a PRKN-dependent manner (PubMed:32484300) and regulates mitochondrial division during mitosis (PubMed:19411255, PubMed:26992161, PubMed:27301544, PubMed:27328748). By suppressing oxidative damage, it ensures the survival of certain postmitotic neurons, including Purkinje cells (By similarity). Essential for normal brain development, particularly in the cerebellum (PubMed:17460227, PubMed:26992161, PubMed:27145208, PubMed:27301544, PubMed:27328748), it also participates in developmentally regulated apoptosis during neural tube formation (By similarity). Rhythmic phosphorylation at Ser-637 is critical for circadian control of mitochondrial ATP production (PubMed:29478834). Overexpression inhibits peroxisomal division.

O00429

Predicted band size: 82 kDa; Observed band size: 82 kDa

Protein A

Non-conjugated

Phosphorylated

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:2000-1:10000; ICC/IF: 1:200-1:1000; ELISA: 1:5000-1:20000; IP: 1:50-1:200;

WB, ICC/IF, IP, ELISA

Liquid

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG