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  3. Primary Antibodies
  4. Monoclonal Antibodies Recombinant Antibodies
  5. Phospho-CD18 (Ser745) Antibody (YA220)

Phospho-CD18 (Ser745) Antibody (YA220)

Cat. No.: HY-P80795
COA
SDS
User Guide for Antibodies Technical Support

Phospho-CD18 (Ser745) Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 85 kDa, targeting to Phospho-CD18 (Ser745). It can be used for WB,IP assays with tag free, in the background of Human, Mouse, Rat.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

Phospho-CD18 (Ser745) Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 85 kDa, targeting to Phospho-CD18 (Ser745). It can be used for WB,IP assays with tag free, in the background of Human, Mouse, Rat.
Background

CD18: This gene encodes an integrin beta chain, which combines with multiple different alpha chains to form different integrin heterodimers. Integrins are integral cell-surface proteins that participate in cell adhesion as well as cell-surface mediated signalling. The encoded protein plays an important role in immune response and defects in this gene cause leukocyte adhesion deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
Tag

Free
Gene ID
SwissProt ID
Synonyms
ITGB2; CD18; MFI7; Integrin beta-2; Cell surface adhesion glycoproteins LFA-1/CR3/p150; 95 subunit beta; Complement receptor C3 subunit beta; CD antigen CD18
Molecular Weight

Predicted band size: 85 kDa; Observed band size: 85 kDa
Purity

affinity purified
Conjugation

Non-conjugated
Modification

Phosphorylated
RRID
Research Field

Tags & Cell Markers
Product Categories

Primary Antibody; Recombinant Rabbit Monoclonal Antibody
Clonality

Recombinant, Monoclonal
Host

Rabbit
Reactivity

Human, Mouse, Rat
Dilution Ratio

WB: 1:500-1:1,000 ;IP: 1:20

  • Western blot analysis of extracts from NIH/3T3 (lane 2(20μg), NIH/3T3 (lane 3(40μg), using Phospho-CD18 (Ser745) Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
Application

WB, IP
Appearance

Liquid
Formulation

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Isotype

IgG
Sensitivity

Endogenous
Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Ser745 of Human CD18.The exact sequence is proprietary to MCE.
Database

Entrez Gene: 3689 Human ; 16414 Mouse ; 309684 Rat

SwissProt: P05107 Human ; P11835 Mouse ;

OMIM: 116920 Human

Documentation
SDS (251 KB)

COA (191 KB)

User Guide for Antibodies (1077 KB)

Phospho-CD18 (Ser745) Antibody (YA220) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Phospho-CD18 (Ser745) Antibody (YA220)
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