Phospho-ATM (S1981) Antibody (YA223)

Phospho-ATM (S1981) Antibody (YA223) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-ATM (S1981).

ATM is a serine/threonine protein kinase that activates checkpoint signaling in response to double-strand breaks (DSBs), apoptosis, and genotoxic stresses such as ionizing radiation, functioning as a DNA damage sensor. It recognizes the substrate consensus sequence [ST]-Q and phosphorylates histone variant H2AX at Ser-139 at DSBs, thereby regulating the DNA damage response mechanism (By similarity). Additionally, ATM plays a role in pre-B cell allelic exclusion by repositioning immunoglobulin heavy chain alleles to enforce clonality. It is involved in signal transduction, cell cycle control, and may act as a tumor suppressor. ATM phosphorylates various substrates, including DYRK2, CHEK2, p53/TP53, BRCA1, etc., modulating their functions. Under genotoxic stress, ATM prevents ubiquitin-mediated degradation of DYRK2 and enhances the DNA damage response by phosphorylating ATF2 and ERCC6. Furthermore, ATM participates in pexophagy by phosphorylating PEX5, promoting its ubiquitination and degradation.

Free

Q13315

Predicted band size: 351 kDa; Observed band size: 370 kDa

Protein A affinity purified.

Nucleus. Cytoplasmic vesicle.

Non-conjugated

Phosphorylated

AB_3102529

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:500-1:1000; ICC/IF: 1:50-1:200; IHC-P: 1:50-1:100; IP: Use at an assay dependent concentration.

WB, ICC/IF, IHC-P, IP

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic phosphopeptide corresponding to residues surrounding Ser1981 of Human ATM.AA range:1940-1989.