PFKFB3 Antibody (YA232)

PFKFB3 Antibody (YA232) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PFKFB3.

PFKFB3 protein is a key player in cellular energy metabolism as it serves a dual function by catalyzing both the synthesis and degradation of fructose 2,6-bisphosphate (F-2,6-BP). F-2,6-BP is a crucial allosteric regulator of glycolysis, exerting control over the rate of glucose utilization for energy production. PFKFB3 acts as a kinase to generate F-2,6-BP, promoting the activation of phosphofructokinase-1 (PFK-1), a rate-limiting enzyme in glycolysis. This results in enhanced glycolytic flux and increased energy production. Conversely, PFKFB3 can also act as a phosphatase to degrade F-2,6-BP, exerting inhibitory effects on PFK-1 and subsequently slowing down glycolysis. The tight regulation of PFKFB3 activity is crucial for maintaining cellular energy homeostasis and has implications in various physiological and pathological processes, including cancer and metabolic disorders. Understanding the intricate regulatory mechanisms governing PFKFB3 can provide insights into therapeutic strategies targeting glycolytic metabolism.

Free

Q16875

Predicted band size: 60 kDa; Observed band size: 60 kDa

Protein A affinity purified.

Cytosol. Nucleoplasm.

Non-conjugated

Unmodified

AB_3102396

Cardiovascular

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:1,000; ICC: 1:50-1:200; IHC-P: 1:50-1:200; FC: 1:50-1:100; IP: Use at an assay dependent concentration.

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  Western blot analysis of extracts from 293T (lane 1) and Hela (lane 2) and HK2 (lane 3) and Jurkat (lane 4) and M-spleen (lane 5) using PFKFB3 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

WB, ICC/IF, IHC-P, FC, IP

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human PFKFB3.AA range:260-520.