HRP-conjugated AffiniPure Goat Anti-Mouse IgG H&L

HRP-conjugated AffiniPure Goat Anti-Mouse IgG H&L is a HRP-conjugated and Goat origined monoclonal antibody, targeting to Mouse IgG antibody. HRP-conjugated AffiniPure Goat Anti-Mouse IgG H&L can binds to the light and heavy chains of Mouse IgG antibodies.

HRP

Unmodified

Secondary antibody

Polyclonal

Goat

Mouse

WB: 1:5,000-1:200,000 ;ELISA: 1:5,000-1:100,000; IHC: 1:500 1:5,000

• 
  Western blot analysis of extracts from Hela(lane 2(20μg), HepG2(lane 3(20μg), Jurkat(lane 4(20μg) ) and NIH3T3(lane 5(20μg) ) using HSP60 (HY-P80184) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80184, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

WB, ELISA, IHC-P

Liquid

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Stored at -20°C for 1 year.Protect from light. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

Endogenous

Synthetic peptide corresponding to Mouse IgG.