PERK Antibody (YA5016)

PERK Antibody (YA5016) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to PERK.

PERK is a metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to various stresses, including the unfolded protein response (UPR). As a key effector of the integrated stress response (ISR) to unfolded proteins, PERK specifically recognizes and binds misfolded proteins, leading to its activation and EIF2S1/eIF-2-alpha phosphorylation. This phosphorylation converts EIF2S1/eIF-2-alpha into a global protein synthesis inhibitor, attenuating cap-dependent translation while initiating preferential translation of ISR-specific mRNAs (e.g., transcriptional activators ATF4 and QRICH1), enabling ATF4- and QRICH1-mediated reprogramming. The PERK-mediated UPR enhances mitochondrial oxidative phosphorylation by promoting ATF4-dependent COX7A2L/SCAF1 expression, increasing respiratory chain supercomplex formation. Unlike other compartments, mitochondria are protected from PERK-mediated UPR due to ATAD3A-mediated inhibition at ER-mitochondria contact sites. Additionally, PERK phosphorylates NFE2L2/NRF2 under stress (by similar), triggering its release from the BCR(KEAP1) complex for nuclear accumulation and activation; contributes to UPR-induced G1 growth arrest via cyclin-D1 (CCND1) loss (by similar); and regulates mitochondrial morphology and function (by similar).

Q9NZJ5

Predicted band size: 125 kDa;

affinity purified.

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human

WB: 1:500-1:2000; ELISA: 1:10000

WB, ELISA

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

Purified recombinant fragment of human PERK expressed in E. Coli.