PD-L2 Antibody (YA3391)

Cat. No.: HY-P81839A: Data Sheet COA
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PD-L2 Antibody (YA3391) is a mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to PD-L2. It can be applicated for WB, ICC/IF, FC, ELISA assays, in the background of human, mouse, rat.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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PD-L2 Antibody (YA3391) Featured Recommendations:

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

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Description	PD-L2 Antibody (YA3391) is a mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to PD-L2. It can be applicated for WB, ICC/IF, FC, ELISA assays, in the background of human, mouse, rat.
Background	The PD-L2 Protein assumes a critical role in the costimulatory signal necessary for T-cell proliferation and IFNG production, operating in a PDCD1-independent manner. While its interaction with PDCD1 can inhibit T-cell proliferation by impeding cell cycle progression and cytokine production, PD-L2 itself is intricately involved in fostering these immune responses. The dynamic interplay between PD-L2 and PDCD1 highlights the regulatory mechanisms at play, emphasizing the protein's dual role in promoting or inhibiting T-cell activation based on its interactions within the immune signaling network.
Gene ID	80380 [NCBI]
SwissProt ID	Q9BQ51
Synonyms	PD-1 ligand 2; PD-L2; PDCD1 ligand 2; B7-DC; CD273; PD L2; PDL2
Molecular Weight	Predicted band size: 31 kDa; Observed band size: 35-70kDa.
Purity	Affinity Purified
Conjugation	Non-conjugated
Modification	Unmodified
Research Field	Immunology
Product Categories	Primary Antibody; Mouse Monoclonal Antibody
Clonality	Monoclonal
Host	Mouse
Reactivity	Human, Mouse, Rat
Dilution Ratio	WB: 1/500-1/1000; IF: 1/50-1/200; FC: 1/50-1/100; ELISA: 1/10000 Western blot analysis was performed on extracts from U2OS (lane 1, 20 μg), 3T3 (lane 2, 20 μg), and Raji (lane 3, 20 μg) using PDL2 Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin,HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Mouse/Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
Application	WB, ICC/IF, FC, ELISA
Appearance	Liquid
Formulation	Supplied in PBS with 0.05% sodium azide.
Storage & Stability	Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping	Shipping with blue ice.
Isotype	IgG2b
Sensitivity	Endogenous
Immunogen	Purified recombinant fragment of human CD273 expressed in E. Coli.
Database	Entrez Gene: 80380 Human ; 58205 Mouse ; 309304 Rat SwissProt: Q9BQ51 Human ; Q9WUL5 Mouse ; OMIM: 605723 Human
Documentation	Select Batch: Data Sheet (234 KB) SDS (251 KB) COA (205 KB) User Guide for Antibodies (1077 KB)

PD-L2 Antibody (YA3391) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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PD-L2 Antibody (YA3391)

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