PARP1 Antibody (YA3733)

PARP1 Antibody (YA3733) is a mouse-derived and non-conjugated IgG1 antibody, targeting to PARP. It can be applicated for WB, FC, ELISA assays, in the background of human.

Cleaved PARP is a poly-ADP-ribosyltransferase that mediates poly-ADP-ribosylation of proteins and plays a key role in DNA repair. It catalyzes ADP-ribosylation of glutamate, aspartate, serine, histidine, or tyrosine residues, with serine ADP-ribosylation being the primary form in response to DNA damage. Specificity for different amino acids is conferred by interacting factors such as HPF1 and NMNAT1. In DNA break repair, Cleaved PARP recognizes and binds DNA breaks in chromatin, recruits HPF1, and promotes serine ADP-ribosylation of histones (e.g., H2BS6ADPr and H3S10ADPr), facilitating chromatin decompaction and repair factor recruitment. It also participates in double-strand break repair, transcription regulation, programmed cell death, membrane repair, adipogenesis, and innate immunity. During apoptosis, Cleaved PARP is cleaved by caspase-3 and caspase-7, translocates to the cytoplasm, and induces AIFM1-mediated apoptosis through auto-poly-ADP-ribosylation. This cleaved form irreversibly binds DNA breaks, interfering with DNA repair and promoting DNA damage-induced apoptosis.

P09874

Predicted band size: 113 kDa; Observed band size: 89 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human

WB: 1/500-1/2000; FC: 1/200-1/400; ELISA: 1/10000

WB, FC, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Synthetic peptide of human PARP, conjugated to KLH.