PAK2 Antibody (YA5004)

PAK2 Antibody (YA5004) is a mouse-derived and non-conjugated monoclonal antibody, targeting to PAK2. It can be applicated for WB, IHC-P, ICC/IF, ELISA assays, in the background of human, monkey.

PAK2 is a serine/threonine protein kinase involved in diverse signaling pathways regulating cytoskeleton dynamics, cell motility, cell cycle progression, apoptosis, and proliferation. It acts as a downstream effector of CDC42/RAC1 GTPases, requiring binding to active CDC42/RAC1 for conformational change and autophosphorylation. Full-length PAK2 promotes cell survival/growth, phosphorylates MAPK4/MAPK6 to activate MAPKAPK5 (regulating F-actin polymerization and migration), and mediates EGF-induced proliferation via JUN phosphorylation. Other substrates include histone H4 (nucleosome assembly), BAD, S6 ribosomal protein, and MBP. PAK2 inhibits CASP7 activity through phosphorylation and performs kinase-independent roles (e.g., mitotic spindle orientation) with ARHGEF7/GIT1. Under apoptotic stimuli (e.g., DNA damage), caspase-cleaved PAK2 generates PAK-2p34, which translocates to the nucleus to drive apoptosis via JNK signaling; caspase-activated PAK2 also phosphorylates MKNK1 to suppress translation.

Q13177

Predicted band size: 58 kDa;

affinity purified.

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Monkey

WB: 1:500-1:2000; IHC: 1:200-1:1000; ICC/IF: 1:200-1:1000; ELISA: 1:10000

WB, IHC-P, ICC/IF, ELISA

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

Purified recombinant fragment of PAKγ expressed in E. Coli.