Nuclear Mitotic Apparatus Protein 1 Antibody (YA6350)

Nuclear Mitotic Apparatus Protein 1 Antibody (YA6350) is a rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NUMA1. It can be applicated for WB, IHC-P, IHC-F, ICC/IF, IF-Tissue, ELISA, FC assays, in the background of human, mouse, rat.

NUMA1 is a microtubule (MT)-binding protein that plays a crucial role in the formation and maintenance of spindle poles and the alignment and segregation of chromosomes during mitotic cell division. It tethers the minus ends of MTs at spindle poles, which is essential for spindle pole establishment and stability. NUMA1 regulates mitotic spindle orientation during metaphase and elongation during anaphase in a dynein-dynactin-dependent manner. During metaphase, it forms a ternary complex with GPSM2 and G(i) alpha proteins to recruit and anchor the dynein-dynactin complex at the cortical regions above spindle poles, ensuring proper spindle orientation. In anaphase, NUMA1 mediates dynein-dynactin accumulation at the polar cortex by binding phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), contributing to spindle elongation and chromosome segregation. It also interacts with other phosphoinositides, including PIP, LPA, and PIP3. NUMA1 is involved in asymmetric cell division, anastral spindle assembly, and positively regulates TNKS localization to spindle poles. As a major nuclear matrix component, it may serve a structural role in non-mitotic contexts. Additionally, NUMA1 is required for epidermal differentiation and hair follicle morphogenesis (by similar).

Q14980

Predicted band size: 238 kDa; Observed band size: 238 kDa

affinity purified.

Nucleus matrix. Chromosome. Cytoplasm, cytoskeleton, spindle pole. Note=Resides in the nuclear matrix during interphases. Dissociates from condensing chromosomes during early prophase, and relocates to the spindle poles via dynein/dynamin association, it remain there until the anaphase onset. Before the complete disintegration of the nuclear lamina. As mitosis progresses it reassociates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident. Isoform Numa-m: Cytoplasm. Note=Mainly clustered at the centrosomal region. Isoform Numa-s: Cytoplasm. Note=Mainly clustered at the centrosomal region.

Non-conjugated

Unmodified

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-2000; IHC-P: 1:100-500; IHC-F: 1:100-500; ICC/IF: 1:50-200; IF: 1:100-500; ELISA: 1:5000-20000; Flow-Cyt: 1μg/Test

WB, IHC-P, IHC-F, ICC/IF, IF-Tissue, ELISA, FC

Liquid

Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

KLH conjugated synthetic peptide derived from human NUMA1