NOTCH4 Antibody (YA4442)

NOTCH4 Antibody (YA4442) is a mouse-derived and non-conjugated IgG1 antibody, targeting to NOTCH4. It can be applicated for ICC/IF, FC, ELISA assays, in the background of human.

NOTCH4 protein functions as a receptor for membrane-bound ligands, including Jagged1, Jagged2, and Delta1, crucial for the regulation of cell-fate determination. Upon ligand activation, the released notch intracellular domain (NICD) forms a transcriptional activator complex with RBPJ/RBPSUH, leading to the activation of genes within the enhancer of split locus. Its role extends to influencing differentiation, proliferation, and apoptotic programs, potentially contributing to the regulation of branching morphogenesis in the developing vascular system. Structurally, NOTCH4 exists as a heterodimer, consisting of a C-terminal fragment (N(TM)) and a N-terminal fragment (N(EC)), likely connected by disulfide bonds. The protein interacts with transcriptional coactivators MAML1, MAML2, and MAML3, facilitating their role in modulating downstream transcriptional processes.

Q99466

Predicted band size: 210 kDa; Observed band size: 230 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human

IF: 1/50-1/200; FC: 1/200-1/400; ELISA: 1/10000

ICC/IF, FC, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Purified recombinant fragment of human NOTCH4 (AA: extra 118-357) expressed in E. Coli.