Notch 2 Antibody (YA6134)

Notch 2 Antibody (YA6134) is a rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Notch 2. It can be applicated for WB, IHC-P, ICC/IF, IP, ELISA assays, in the background of human, mouse, rat.

NOTCH2 protein functions as a receptor for membrane-bound ligands, including Jagged-1 (JAG1), Jagged-2 (JAG2), and Delta-1 (DLL1), playing a pivotal role in regulating cell-fate determination. Upon ligand activation, NOTCH2 undergoes cleavage, releasing the Notch intracellular domain (NICD), which forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes within the enhancer of split locus. This activation influences cellular differentiation, proliferation, and apoptotic programs. NOTCH2 is implicated in bone remodeling and homeostasis, collaborating with RELA/p65 to enhance NFATc1 promoter activity and positively regulate RANKL-induced osteoclast differentiation. Additionally, it positively regulates self-renewal in liver cancer cells. NOTCH2 exists as a heterodimer composed of a C-terminal fragment (N(TM)) and an N-terminal fragment (N(EC)), likely linked by disulfide bonds. It interacts with various proteins, including transcriptional coactivators MAML1, MAML2, and MAML3, as well as factors such as HIF1AN, TCIM, and FBXW7, implicating its involvement in diverse cellular processes and regulatory pathways. Interactions with MINAR1, NOTCH2NL, MDK, and MINAR2 further contribute to the intricate network of NOTCH2 signaling.

Q04721

Predicted band size: 265 kDa; Observed band size: 120 kDa

Protein A

Non-conjugated

Unmodified

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Monoclonal

Rabbit

Human, Mouse, Rat

IHC: 1:100-1:1000; WB: 1:1000-1:5000; ICC/IF: 1:200-1:1000; ELISA: 1:5000-1:20000; IP: 1:50-1:200;

WB, IHC-P, ICC/IF, IP, ELISA

Liquid

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG