NF-KB p105/p50 Antibody (YA699)

Cat. No.: HY-P80244: Data Sheet COA
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NF-KB p105/p50 Antibody (YA699) is a Mouse-derived and non-conjugated IgM monoclonal antibody, targeting to NF-KB p105/p50.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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NF-KB p105/p50 Antibody (YA699) Featured Recommendations:

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

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Description	NF-KB p105/p50 Antibody (YA699) is a Mouse-derived and non-conjugated IgM monoclonal antibody, targeting to NF-KB p105/p50.
Background	NFKB1 protein serves as a pivotal component of the pleiotropic transcription factor NF-kappa-B, found ubiquitously across various cell types. This transcription factor acts as a central mediator in signal transduction pathways associated with diverse biological processes, including inflammation, immunity, differentiation, cell growth, tumorigenesis, and apoptosis. NFKB1 participates in homo- or heterodimeric complexes, with the p65-p50 heterodimer being the most prevalent. These dimers selectively bind to kappa-B sites in target gene DNA, exhibiting distinct preferences and affinities. NF-kappa-B regulation involves intricate post-translational modifications, subcellular compartmentalization, and interactions with cofactors or corepressors. In an inactive state, NF-kappa-B complexes associate with NF-kappa-B inhibitor (I-kappa-B) family members in the cytoplasm. Activation ensues through phosphorylation and degradation of I-kappa-B, liberating active NF-kappa-B complexes for nuclear translocation. NFKB1 exhibits dual functions, contributing to cytoplasmic retention of NF-kappa-B proteins and generating the active p50 subunit through cotranslational processing. This processing, mediated by the proteasome, ensures the independent functionality of p50 and p105. The p50 subunit binds to kappa-B consensus sequences in the enhancer regions of genes involved in immune response and acute phase reactions. Additionally, NFKB1, in association with MAP3K8, represses MAP3K8-induced MAPK signaling, with active MAP3K8 released through proteasome-dependent degradation of NFKB1/p105.
Tag	Free
Gene ID	4790 [NCBI]
SwissProt ID	P19838
Synonyms	DKFZp686C01211 antibody; DNA binding factor KBF1 antibody; DNA binding factor KBF1 EBP1 antibody; DNA-binding factor KBF1 antibody; EBP 1 antibody; EBP-1 antibody; EBP1 antibody; KBF1 antibody; MGC54151 antibody; NF kappa B antibody; NF kappaB antibody; NF kappabeta antibody; NF kB1 antibody; NFkappaB antibody; NFKB 1 antibody; NFKB p105 antibody; NFKB p50 antibody; Nfkb1 antibody; NFKB1_HUMAN antibody; Nuclear factor kappa B DNA binding subunit antibody; Nuclear factor kappa-B, subunit 1 antibody; Nuclear factor NF kappa B p105 subunit antibody; Nuclear factor NF kappa B p50 subunit antibody; Nuclear factor NF-kappa-B p50 subunit antibody; Nuclear factor of kappa light chain gene enhancer in B cells 1 antibody; Nuclear factor of kappa light polypeptide gene enhancer in B cells 1 antibody; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 antibody; p105 antibody; p50 antibody; p84/NF-kappa-B1 p98 antibody; Transcription factor NFKB1 antibody;
Molecular Weight	Predicted band size: 105 kDa; Observed band size: 50/120 kDa
Purity	Protein L affinity purified.
Subcellular Location	Nucleus. Cytoplasm.
Conjugation	Non-conjugated
Modification	Unmodified
RRID	AB_3102375
Research Field	Cell Biology
Product Categories	Primary Antibody; Mouse Monoclonal Antibody
Clonality	Monoclonal
Host	Mouse
Reactivity	Human, Mouse
Dilution Ratio	WB: 1:1,000-1:2,000 ;ICC: 1:200 ;IHC-P: 1:200-1:500
Application	WB, ICC/IF, IHC-P
Appearance	Liquid
Formulation	Supplied in 1*PBS (pH7.4), 0.2% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.
Storage & Stability	Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping	Shipping with blue ice.
Isotype	IgM
Sensitivity	Endogenous
Immunogen	Synthetic peptide corresponding to Human NF-kB p105/p50.AA range:317-366.
Database	Entrez Gene: 4790 Human ; 18033 Mouse ; SwissProt: P19838 Human ; P25799 Mouse ; OMIM: 616576 Human
Documentation	Select Batch: Data Sheet (235 KB) SDS (251 KB) COA (191 KB) User Guide for Antibodies (1077 KB)

NF-KB p105/p50 Antibody (YA699) Related Classifications

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Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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NF-KB p105/p50 Antibody (YA699)

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