MYCN Antibody (YA3636)

MYCN Antibody (YA3636) is a mouse-derived and non-conjugated IgG1 antibody, targeting to MYCN. It can be applicated for IHC-P, FC, ELISA assays, in the background of human.

MYCN, a critical player in neuroblastoma cells, exerts positive regulatory control over the transcription of MYCNOS. This regulatory function is contingent on efficient DNA binding, necessitating dimerization with another basic helix-loop-helix (bHLH) protein. Notably, MYCN forms a heterodimer with MAX for effective DNA binding. The intricate network of interactions involves KDM5A, KDM5B, and HUWE1, indicating its involvement in various cellular processes. Furthermore, MYCN engages in phospho-independent interactions with AURKA, triggering AURKA activation, while also interacting with MYCNOS. The competitive dynamics between AURKA and FBXW7 for binding to unphosphorylated MYCN unveil additional layers of regulatory complexity, emphasizing the multifaceted nature of MYCN's involvement in cellular pathways.

P04198

Predicted band size: 50 kDa; Observed band size: 50 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human

IHC: 1/200-1/1000; FC: 1/200-1/400; ELISA: 1/10000

IHC-P, FC, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Purified recombinant fragment of human MYCN (AA: 337-464) expressed in E. Coli.