MuSK Antibody (YA4980)

MuSK Antibody (YA4980) is a mouse-derived and non-conjugated monoclonal antibody, targeting to MuSK. It can be applicated for IHC-P, ICC/IF, ELISA assays, in the background of human.

The MUSK protein, a receptor tyrosine kinase, holds a pivotal role in the formation and maintenance of the neuromuscular junction (NMJ), the synapse connecting motor neurons to skeletal muscles. Upon AGRIN recruitment by LRP4 to the MUSK signaling complex, MUSK undergoes phosphorylation and activation, regulating NMJ formation by influencing gene expression in subsynaptic nuclei, orchestrating actin cytoskeleton reorganization, and clustering acetylcholine receptors (AChR) in the postsynaptic membrane. ABL1 and Src family kinases, activated by MUSK, may further regulate AChR phosphorylation and clustering. The ternary complex formed by MUSK, DVL1, and PAK1 is crucial for AChR clustering regulation. Additionally, MUSK positively regulates Rho family GTPases through FNTA, mediating the phosphorylation of FNTA and promoting the activation of RAC1, a key regulator of the actin cytoskeleton and gene expression. DNAJA3, acting downstream of MUSK, is another effector in the MUSK signaling pathway. Beyond the neuromuscular junction, MUSK may also contribute to cholinergic responses, synaptic plasticity, and memory formation within the central nervous system.

O15146

affinity purified.

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human

IHC: 1:200-1:1000; ICC/IF: 1:200-1:1000; ELISA: 1:10000

IHC-P, ICC/IF, ELISA

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

Purified recombinant extracellular fragment of human MuSK (aa24-209) fused with hIgGFc tag expressed in HEK293 cell line.