MSH6 Antibody (YA5611)

MSH6 Antibody (YA5611) is a mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MSH6. It can be applicated for IHC-P, WB, ICC/IF, ELISA assays, in the background of human.

MSH6 is a component of the post-replicative DNA mismatch repair system (MMR). It heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches to initiate DNA repair. Upon binding, MutS alpha bends the DNA helix and shields approximately 20 base pairs, while recognizing single base mismatches and dinucleotide insertion-deletion loops (IDLs) in the DNA. After mismatch binding, MutS alpha forms a ternary complex with the MutL alpha heterodimer, which is thought to direct downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similarly to a molecular switch: mismatched DNA triggers ADP→ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also participate in DNA homologous recombination repair. It is recruited to chromatin during G1 and early S phase via its PWWP domain, which specifically binds trimethylated 'Lys-36' of histone H3 (H3K36me3). Early recruitment to chromatin destined for replication allows rapid identification of mismatch repair to initiate the DNA mismatch repair reaction.

P52701

Predicted band size: 153 kDa; Observed band size: 180 kDa

Protein G

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human

IHC: 1:100-500; WB: 1:500-2000; ICC/IF: 1:100-500; ELISA: 1:1000-5000

IHC-P, WB, ICC/IF, ELISA

Liquid

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Synthesized peptide derived from human MutS Protein Homolog 6(MSH6) AA range: 900-1000