MSH2 Antibody (YA5654)

MSH2 Antibody (YA5654) is a mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MSH2. It can be applicated for IHC-P, WB, ICC/IF, ELISA assays, in the background of human.

MSH2 protein is a key component of the post-replicative DNA mismatch repair system (MMR), crucial for maintaining genomic stability. Forming two distinct heterodimers, MutS alpha (MSH2-MSH6) and MutS beta (MSH2-MSH3), MSH2 recognizes and binds to DNA mismatches, initiating repair processes. MutS alpha targets single base mismatches and dinucleotide insertion-deletion loops (IDL), while MutS beta recognizes larger insertion-deletion loops. Upon mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, directing downstream MMR events such as strand discrimination, excision, and resynthesis. MSH2's ATPase activity is crucial for mismatch repair, acting as a molecular switch that undergoes ADP-->ATP exchange upon mismatch recognition, enabling its conversion into a sliding clamp for hydrolysis-independent diffusion along DNA. Additionally, MSH2 may participate in DNA homologous recombination repair and modulate UV-B-induced cell cycle regulation and apoptosis in melanocytes. As part of the DNA MMR complex, MSH2 collaborates with MSH3, MSH6, PMS1, and MLH1, forming a highly coordinated system essential for genome integrity. MSH2 engages in various protein interactions, including those with MCM9, MCM8, EXO1, ATR, SLX4/BTBD12, and SMARCAD1, highlighting its multifaceted role in maintaining DNA fidelity and participating in broader cellular processes.

P43246

Predicted band size: 105 kDa; Observed band size: 105 kDa

Protein G

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human

IHC: 1:100-500; WB: 1:500-2000; ICC/IF: 1:100-500; ELISA: 1:1000-5000

IHC-P, WB, ICC/IF, ELISA

Liquid

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Synthesized peptide derived from human MutS Protein Homolog 2(MSH2) AA range: 600-700