MRE11 Antibody (YA4059)

MRE11 Antibody (YA4059) is a mouse-derived and non-conjugated IgG2b antibody, targeting to MRE11. It can be applicated for WB, IHC-P, FC, ELISA assays, in the background of human, mouse, monkey, rat.

Mre11 is the core component of the MRN complex, playing a central role in double-strand break (DSB) repair, DNA recombination, telomere integrity maintenance, and meiosis. The MRN complex repairs DSBs via homologous recombination (HR), primarily during the S and G2 phases, by (1) mediating end resection of damaged DNA to generate single-stranded DNA (ssDNA), a key initial step in HR, and (2) recruiting other repair factors and activating ATM and ATR in response to DNA damage. MRE11 possesses both single-strand endonuclease and double-strand-specific 3'-5' exonuclease activities, collaborating with RBBP8/CtIP at DSB sites to initiate end resection. It first cleaves the 5' strand endonucleolytically to suppress non-homologous end joining (NHEJ) and license HR, then generates an ssDNA gap via 3' to 5' exonucleolytic degradation to enable EXO1- and DNA2-mediated long-range resection. RBBP8/CtIP enhances MRE11's endonuclease activity to clear protein-DNA adducts (By similar). The MRN complex also activates ATM and ATR for DNA damage signaling and processes R-loops. In telomeres, it may modulate t-loop formation. MRE11 contains two DNA-binding domains (DBDs) for binding both ssDNA and dsDNA.

P49959

Predicted band size: 81 kDa; Observed band size: 81 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human, Mouse, Monkey, Rat

WB: 1/500-1/2000; IHC: 1/200-1/1000; FC: 1/200-1/400; ELISA: 1/10000

WB, IHC-P, FC, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG2b

Purified recombinant fragment of human MRE11 (AA: 182-582) expressed in E. Coli.