MiTF Antibody (YA6306)

MiTF Antibody (YA6306) is a rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MiTF. It can be applicated for WB, IHC-P, ICC/IF, IP, ELISA assays, in the background of human, mouse, rat.

MiTF is a transcription factor that serves as a master regulator of melanocyte survival, differentiation, and melanosome biogenesis. It binds to M-boxes (5'-TCATGTG-3') and E-boxes (5'-CACGTG-3') in the promoters of pigmentation genes like tyrosinase (TYR). Under nutrient-rich conditions, MTOR-mediated phosphorylation inactivates MiTF, while starvation or lysosomal stress triggers its dephosphorylation and transcriptional activation via MTOR inhibition. MiTF critically regulates melanocyte development by controlling TYR and TYRP1 expression and is involved in differentiating neural crest-derived melanocytes, mast cells, osteoclasts, and retinal pigment epithelium. by similar: MiTF's role is conserved across diverse cell-type differentiation processes.

O75030

Predicted band size: 58 kDa; Observed band size: 58 kDa

Protein A

Non-conjugated

Unmodified

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Monoclonal

Rabbit

Human, Mouse, Rat

IHC: 1:200-500; WB: 1:1000-5000; ICC/IF: 1:200-1000; ELISA: 1:5000-20000; IP: 1:50-200

WB, IHC-P, ICC/IF, IP, ELISA

Liquid

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG