METTL3 Antibody (YA294)

METTL3 Antibody (YA294) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to METTL3.

The METTL3-METTL14 heterodimer functions as an N6-methyltransferase complex that catalyzes adenosine methylation (m6A) at the N(6) position of RNAs, regulating processes including circadian rhythm, stem cell differentiation, DNA damage response, T-cell differentiation, and primary miRNA processing. METTL3 serves as the catalytic core, mediating m6A modifications at 5'-[AG]GAC-3' consensus sites of mRNAs to influence stability, processing, translation efficiency, and editing. m6A destabilizes pluripotency transcripts to promote embryonic stem cell differentiation (by similar), accelerates nuclear mRNA processing as an early circadian pace-setter (by similar), and modulates hepatic lipid metabolism (PubMed:30428350), spermatogenesis (by similar), and oogenesis (by similar). During DNA damage response, METTL3 rapidly generates m6A on poly(A) transcripts to recruit POLK (PubMed:28297716). It promotes T-cell differentiation by destabilizing SOCS family transcripts (by similar) and suppresses type I interferon response via IFNB methylation (PubMed:30559377). METTL3 also methylates Xist RNA to facilitate random X-chromosome inactivation (PubMed:27602518), regulates cortical neurogenesis by degrading neural development transcripts (by similar), and marks pri-miRNAs for DGCR8 processing (PubMed:25799998). Independently of methyltransferase activity, it enhances translation initiation (PubMed:27117702), potentially contributing to cancer proliferation. During SARS-CoV-2 infection, viral RNA m6A modifications dampen RIGI-mediated innate immunity (PubMed:33961823).

Free

Q86U44

Predicted band size: 64 kDa；Observed band size: 72 kDa

Protein A affinity purified.

Nucleus, Nucleus speckle, Cytoplasm.

Non-conjugated

Unmodified

AB_3102359

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:1,000 ;IHC-P: 1:50-1:100 ;FC: 1:50-1:100

• 
  Western blot analysis of extracts from HK2 (lane 1) and Hela (lane 2) and M-heart (lane 3) and Jurkat (lane 4) using METTL3 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

WB, IHC-P, FC

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human METTL3.AA range:1-300.