MEK4 Antibody (YA6036)

MEK4 Antibody (YA6036) is a rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MEK4. It can be applicated for WB, ICC/IF, IP, ELISA assays, in the background of human, mouse, rat.

MEK4 is a dual specificity protein kinase that functions as an essential component of the MAP kinase signal transduction pathway. It plays a critical role in the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. Together with MAP2K7/MKK7, MEK4 is one of the only known kinases that directly activates the stress-activated protein kinases/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2, and MAPK10/JNK3. Both MAP2K4/MKK4 and MAP2K7/MKK7 activate JNKs through phosphorylation but differ in their preference for phosphorylation sites within the Thr-Pro-Tyr motif: MAP2K4 preferentially phosphorylates the Tyr residue, while MAP2K7/MKK7 targets the Thr residue. Phosphorylation of the Thr residue by MAP2K7/MKK7 appears to be a prerequisite for JNK activation, at least in response to pro-inflammatory cytokines, whereas other stimuli activate both MAP2K4/MKK4 and MAP2K7/MKK7 to synergistically phosphorylate JNKs. MEK4 is required for maintaining peripheral lymphoid homeostasis. The MKK/JNK signaling pathway is also involved in the mitochondrial death signaling pathway, including cytochrome c release, leading to apoptosis. Unlike MAP2K7/MKK7, which exclusively activates JNKs, MAP2K4/MKK4 additionally activates p38 MAPKs (MAPK11, MAPK12, MAPK13, and MAPK14).

P45985

Predicted band size: 44 kDa; Observed band size: 44 kDa

Protein A

Non-conjugated

Unmodified

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:2000-1:10000; ICC/IF: 1:200-1:1000; ELISA: 1:5000-1:20000; IP: 1:50-1:200;

WB, ICC/IF, IP, ELISA

Liquid

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG