MEK1 Antibody (YA300)

MEK1 Antibody (YA300) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MEK1.

MEK1 protein, a pivotal component of the MAP kinase signal transduction pathway, plays a crucial role in transducing extracellular signals into intracellular responses. Upon binding of ligands such as growth factors and cytokines to their cell-surface receptors, MEK1 is activated as part of the RAS-RAF1-MEK1/2-MAPK/ERK cascade. MEK1, along with MAP2K2/MEK2, catalyzes the dual phosphorylation of threonine and tyrosine residues in the activation loop of ERK1 and ERK2, thereby initiating downstream signaling events. This cascade regulates diverse biological functions, including cell growth, adhesion, survival, and differentiation. In addition to its role in the transcriptional and cytoskeletal regulation, the MAPK/ERK pathway impacts peroxisome proliferator-activated receptor gamma (PPARG), contributing to cellular processes such as differentiation and apoptosis. Furthermore, MEK1's involvement in endosomal dynamics and Golgi apparatus fragmentation during mitosis underscores its versatility in coordinating intricate cellular responses.

Free

Q02750

Predicted band size: 43 kDa; Observed band size: 45 kDa

Protein A affinity purified.

Cytoplasm, Nucleus, Membrane, Cytoskeleton.

Non-conjugated

Unmodified

AB_3102355

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse

WB: 1:500-1:2,000; ICC/IF: 1:50-1:200; IHC-P: 1:50-1:200; FC: 1:50-1:100; IP: Use at an assay dependent concentration.

• 
  Western blot analysis of extracts from Hela (lane 1) and Jurkat (lane 2) using MEK1 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

WB, ICC/IF, IHC-P, IP, FC

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide within N-terminal human MEK1.