MCM2 Antibody (YA5651)

MCM2 Antibody (YA5651) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MCM2.

MCM2 is a core component of the MCM2-7 complex (MCM complex), the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. It forms part of the CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication and serves as the scaffold for replisome assembly (by similar: PubMed:32453425, PubMed:34694004, PubMed:34700328, PubMed:35585232). The ATPase active sites in the MCM2-7 ring are generated through interfaces of adjacent subunits, with a conserved arginine finger motif acting in trans on the Walker A box ATP-binding site of neighboring subunits, though the six ATPase sites likely contribute asymmetrically to helicase activity (by similar: PubMed:32453425). MCM2 is required for S-phase entry and cell division (by similar: PubMed:8175912) and also regulates terminally differentiated cochlear hair cell development and apoptosis induction (by similar: PubMed:26196677).

P49736

Predicted band size: 120 kDa; Observed band size: 130 kDa

Protein G

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Mouse,

IHC: 1:100-500; WB: 1:500-2000; ICC/IF: 1:100-500; ELISA: 1:1000-5000

IHC-P, WB, ICC/IF, ELISA

Liquid

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Synthesized peptide derived from human MCM2 AA range: 300-400