MCM2 Antibody (YA4750)

MCM2 Antibody (YA4750) is a mouse-derived and non-conjugated monoclonal antibody, targeting to MCM2. It can be applicated for WB, IHC-P, ICC/IF, FC, ELISA assays, in the background of human, mouse, rat, monkey.

MCM2 is a core component of the MCM2-7 complex (MCM complex), the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. It forms part of the CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication and serves as the scaffold for replisome assembly (by similar: PubMed:32453425, PubMed:34694004, PubMed:34700328, PubMed:35585232). The ATPase active sites in the MCM2-7 ring are generated through interfaces of adjacent subunits, with a conserved arginine finger motif acting in trans on the Walker A box ATP-binding site of neighboring subunits, though the six ATPase sites likely contribute asymmetrically to helicase activity (by similar: PubMed:32453425). MCM2 is required for S-phase entry and cell division (by similar: PubMed:8175912) and also regulates terminally differentiated cochlear hair cell development and apoptosis induction (by similar: PubMed:26196677).

P49736

Predicted band size: 102 kDa;

affinity purified.

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Mouse, Rat, Monkey

WB: 1:500-1:2000; IHC: 1:200-1:1000; FC: 1:200-1:400; ELISA: 1:10000; ICC/IF: 1:50-200

WB, IHC-P, ICC/IF, FC, ELISA

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

Purified recombinant fragment of human BM28 expressed in E. Coli.