LYN Antibody (YA5569)

LYN Antibody (YA5569) is a mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to LYN. It can be applicated for WB, ICC/IF, ELISA assays, in the background of human, mouse, rat.

Lyn Protein, a non-receptor tyrosine-protein kinase, serves as a central mediator in transmitting signals from cell surface receptors, playing a crucial role in regulating innate and adaptive immune responses, hematopoiesis, growth factor and cytokine responses, integrin signaling, and the cellular response to DNA damage and genotoxic agents. Acting primarily as a negative regulator but also functioning as an activator depending on the cellular context, Lyn Protein is indispensable for initiating and terminating B-cell responses, influencing B-cell differentiation, proliferation, survival, apoptosis, and immune self-tolerance. It acts downstream of various immune receptors, including the B-cell receptor, CD79A, CD79B, CD5, CD19, CD22, FCER1, FCGR2, FCGR1A, TLR2, and TLR4, contributing to the inflammatory response to bacterial lipopolysaccharide. Lyn Protein also mediates responses to cytokines and growth factors in diverse hematopoietic cell types, playing a crucial role in integrin signaling. Its regulatory role encompasses cell proliferation, survival, differentiation, migration, adhesion, degranulation, and cytokine release. Lyn Protein down-regulates signaling pathways through phosphorylation of immunoreceptor tyrosine-based inhibitory motifs (ITIM), facilitating the recruitment of phosphatases such as PTPN6/SHP-1, PTPN11/SHP-2, and INPP5D/SHIP-1 to modulate signaling by dephosphorylation of kinases and substrates. Lyn Protein's multifaceted kinase activity extends to phosphorylating various substrates, including BTK, CBL, CD5, CD19, CD72, CD79A, CD79B, CSF2RB, DOK1, HCLS1, LILRB3/PIR-B, MS4A2/FCER1B, SYK, and TEC, showcasing its intricate involvement in diverse signaling cascades. It is also involved in the phosphorylation of SIRPA, PTPN6/SHP-1, PTPN11/SHP-2, INPP5D/SHIP-1, BCR-ABL fusion protein, FER, KIT, LPXN, SCIMP, CLNK, BCAR1/CAS, and NEDD9/HEF1, illustrating its extensive regulatory capacity in various cellular processes.

P07948

Predicted band size: 61 kDa; Observed band size: 61 kDa

Protein G

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Mouse, Rat

WB: 1:500-2000; ICC/IF: 1:100-500; ELISA: 1:1000-5000

WB, ICC/IF, ELISA

Liquid

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Synthesized peptide derived from human Lyn. AA range: 450-512