KDM1/LSD1 Antibody (YA3547)

KDM1/LSD1 Antibody (YA3547) is a mouse-derived and non-conjugated IgG1 antibody, targeting to LSD1/AOF2. It can be applicated for WB, IHC-P, ELISA assays, in the background of human, mouse, monkey.

LSD1, a histone demethylase, exhibits the unique capability to demethylate both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, functioning as a versatile coactivator or corepressor depending on the cellular context. Acting through FAD-mediated oxidation and subsequent imine hydrolysis, LSD1 can demethylate mono- and di-methylated H3K4me, thus contributing to the regulation of epigenetic transcriptional activation. In the absence of RCOR1/CoREST, LSD1 alone is unable to demethylate H3K4me on nucleosomes. Additionally, LSD1 serves as a coactivator for androgen receptor (AR)-dependent transcription, participating in the demethylation of H3K9me, a marker associated with epigenetic transcriptional repression. Moreover, LSD1 plays a pivotal role in suppressing neuronal genes and orchestrates the repression of E-cadherin/CDH1, CDN7, and KRT8 through its involvement in the RCOR/GFI/KDM1A/HDAC complex. Beyond histone demethylation, LSD1's diverse interactions extend to demethylating p53/TP53, stabilizing DNMT1, and modifying SOX2. The intricate regulatory role of LSD1 spans embryogenesis, gastrulation, and multilineage blood cell development, emphasizing its significance in shaping the epigenetic landscape and influencing various cellular processes.

O60341

Predicted band size: 93 kDa; Observed band size: 93 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human, Mouse, Monkey

WB: 1/500-1/2000; IHC: 1/200-1/1000; ELISA: 1/10000

WB, IHC-P, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Purified recombinant fragment of human LSD1 expressed in E. Coli.