LRRK2 Antibody (YA4329)

LRRK2 Antibody (YA4329) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to LRRK2.

LRRK2 is a serine/threonine-protein kinase that phosphorylates a broad range of proteins involved in neuronal plasticity, innate immunity, autophagy, and vesicle trafficking. It serves as a key regulator of RAB GTPases by controlling their GTP/GDP exchange and interaction partners through phosphorylation. LRRK2 phosphorylates RAB3A, RAB3B, RAB3C, RAB3D, RAB5A, RAB5B, RAB5C, RAB8A, RAB8B, RAB10, RAB12, RAB29, RAB35, and RAB43. By phosphorylating RAB8A at Thr-72, it modulates RAB3IP-catalyzed GDP/GTP exchange and inhibits RAB8A binding to GDI1/GDI2. Additionally, LRRK2 promotes primary ciliogenesis via phosphorylation of RAB8A and RAB10, enhancing SHH signaling in the brain. Together with RAB29, it facilitates retromer-dependent retrograde trafficking between lysosomes and the Golgi apparatus. LRRK2 regulates neuronal morphology, synaptic vesicle trafficking, and endoplasmic reticulum exit site (ERES) organization. It positively regulates autophagy through calcium-dependent activation of the CaMKK/AMPK pathway and phosphorylates PRDX3 and APP (promoting AICD production and nuclear translocation). In innate immunity, LRRK2 enhances RIPK2 activation downstream of NOD1/NOD2. Independently of its kinase activity, it inhibits MAPT proteasomal degradation, promoting oligomerization and secretion. Its Roc domain exhibits GTPase activity, modulating LRRK2 kinase function. Recruited by RAB29/RAB7L1 to overloaded lysosomes, LRRK2 stabilizes RAB8A and RAB10 via phosphorylation, facilitating lysosomal content release and suppressing lysosomal enlargement.

Q5S007

Predicted band size: 286 kDa; Observed band size: 280 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human

IF: 1/50-1/200; FC: 1/200-1/400; ELISA: 1/10000

ICC/IF, FC, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Purified recombinant fragment of human LRRK2 (AA: 300-530) expressed in E. Coli.