Proteasome 20S LMP7 Antibody (YA4953)

Proteasome 20S LMP7 Antibody (YA4953) is a mouse-derived and non-conjugated monoclonal antibody, targeting to LMP7A. It can be applicated for WB, IHC-P, ICC/IF, ELISA assays, in the background of human, rat.

LMP7A is a subunit of the proteasome involved in antigen processing to generate class I binding peptides. By replacing PSMB5, it enhances the immunoproteasome's ability to cleave model peptides after hydrophobic and basic residues. LMP7A also participates in generating spliced peptides through the ligation of two non-contiguous proteasomal cleavage products and acts as a major component in interferon gamma-induced sensitivity. During apoptosis, it plays a key role by degrading the apoptotic inhibitor MCL1 and may be involved in the inflammatory response pathway. In cancer cells, the substitution of isoform 1 (E2) by isoform 2 (E1) results in immunoproteasome deficiency. Additionally, LMP7A is required for the differentiation of preadipocytes into adipocytes. by similar: The proteasome is a multicatalytic protease complex capable of cleaving peptides adjacent to specific residues (Arg, Phe, Tyr, Leu, Glu) at neutral or slightly basic pH and exhibits ATP-dependent proteolytic activity.

P28062

Predicted band size: 30 kDa;

affinity purified.

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Rat

WB: 1:500-1:2000; IHC: 1:200-1:1000; ICC/IF: 1:200-1:1000; ELISA: 1:10000

WB, IHC-P, ICC/IF, ELISA

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

Purified recombinant fragment of human LMP7A expressed in E. Coli.