LKB1 Antibody (YA6032)

LKB1 Antibody (YA6032) is a rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to LKB1. It can be applicated for WB, ICC/IF, IP, ELISA assays, in the background of human, mouse, rat.

LKB1 is a tumor suppressor serine/threonine-protein kinase that regulates AMP-activated protein kinase (AMPK) family members, playing pivotal roles in cell metabolism, polarity, apoptosis, and DNA damage response. It activates AMPK family proteins (e.g., PRKAA1, PRKAA2, BRSK1, BRSK2) via T-loop phosphorylation and also targets non-AMPK proteins like STRADA, PTEN, and potentially p53/TP53. As a master upstream regulator of AMPK, LKB1 phosphorylates PRKAA1/PRKAA2 to inhibit growth signaling under energy stress, maintain hepatic glucose homeostasis, induce nutrient-deprivation autophagy, and mediate germinal center B-cell differentiation. In cell polarity, LKB1 remodels the actin cytoskeleton by activating BRSK1/BRSK2 for axon initiation in cortical neurons. During DNA damage response, LKB1 interacts with p53/TP53 at the CDKN1A/WAF1 promoter for transcriptional activation and may indirectly phosphorylate p53 via NUAK1. Collaborating with NUAK1, LKB1 phosphorylates CDKN1A post-UV radiation to facilitate its degradation for efficient DNA repair. It also mediates p53-dependent apoptosis through mitochondrial translocation and participates in spermiogenesis.

Q15831

Predicted band size: 49 kDa; Observed band size: 60 kDa

Protein A

Non-conjugated

Unmodified

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:2000-1:10000; ICC/IF: 1:200-1:1000; ELISA: 1:5000-1:20000; IP: 1:50-1:200;

WB, ICC/IF, IP, ELISA

Liquid

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG