Lipin 1 Antibody (YA1909)

Lipin 1 Antibody (YA1909) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Lipin 1.

The related gene encodes a magnesium-ion-dependent phosphatidic acid phosphohydrolase enzyme that catalyzes the penultimate step in triglyceride synthesis including the dephosphorylation of phosphatidic acid to yield diacylglycerol. Expression of The related gene is required for adipocyte differentiation and it also functions as a nuclear transcriptional coactivator with some peroxisome proliferator-activated receptors to modulate expression of other related genes involved in lipid metabolism. Mutations in The related gene are associated with metabolic syndrome, type 2 diabetes, acute recurrent rhabdomyolysis, and autosomal recessive acute recurrent myoglobinuria (ARARM). The related gene is also a candidate for several human lipodystrophy syndromes.

Q14693

Predicted band size: 99 kDa; Observed band size: 130 kDa

Affinity Chromatography

Non-conjugated

Unmodified

Cardiovascular

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1/500-1/1000;IHC: 1/50-1/100;IF: 1/50-1/200;IP: 1/30;FC: 1/50-1/100

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  Western blot analysis was performed on extracts from Jurkat (lane 1, 15 μg), HepG2 (lane 2, 15 μg), LNcap (lane 3, 15 μg), A549 (lane 4, 15 μg), and MOLT-4 (lane 5, 15 μg) using Lipin 1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80487, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

WB, IHC-P, ICC/IF, IP, FC

Liquid

Supplied in rabbit IgG in 50 mM Tris-Glycine, 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA, pH 7.4.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human Lipin 1