LINE-1 ORF1p Antibody (YA3039)

LINE-1 ORF1p Antibody (YA3039) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA3039), targeting LINE 1 ORF1p, with a predicted molecular weight of 40 kDa (observed band size: 40 kDa). LINE-1 ORF1p Antibody (YA3039) can be used for WB, IP experiment in human background.

LINE 1 ORF1p is a nucleic acid-binding protein essential for the retrotransposition of LINE-1 elements in the genome. It functions as a nucleic acid chaperone, binding its own transcript and preferentially mobilizing the transcript from which it is encoded.

Q9UN81

Predicted band size: 40 kDa; Observed band size: 40 kDa

Affinity Purified

Non-conjugated

Unmodified

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1/500-1/1000;IP: 1/20

• 
  Western blot analysis was performed on extracts from Hela (lane 1, 20 μg), A549 (lane 2, 20 μg), U2OS (lane 3, 20 μg), and K562 (lane 4, 20 μg) using LINE 1 ORF1p Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit/Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

WB, IP

Liquid

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Recombinant protein of human LINE-1 ORF1p