LIN28 Antibody (YA1473)

LIN28 Antibody (YA1473) is a non-conjugated IgG antibody, targeting LIN28, with a predicted molecular weight of 23 kDa. LIN28 Antibody (YA1473) can be used for WB, IHC-P, ICC/IF, IP, FC experiment in human background.

LIN28 is an RNA-binding protein that inhibits the processing of pre-let-7 miRNAs and regulates the translation of mRNAs involved in developmental timing, pluripotency, and metabolism (e.g., binding IGF2 mRNA, MYOD1 mRNA). It recognizes G-quartet (G4) structural features in miRNA/mRNA targets, acting as a 'translational enhancer' to drive specific mRNAs to polysomes and boost protein synthesis. By associating with the translational machinery, LIN28 increases initiation events per mRNA and stabilizes mRNAs indirectly. It recruits TUT4/TUT7 uridylyltransferases to uridylate pre-miRNAs (e.g., let-7, miR-143), leading to their Dicer-blocked degradation, thereby maintaining embryonic stem cell pluripotency (similar mechanisms apply to miR107, miR-200c). Localized near the ER, LIN28 suppresses translation of ER-targeted mRNAs (e.g., transmembrane/secretory proteins) but enhances translation of metabolic enzymes (e.g., PFKP, PDHA1, SDHA), promoting glycolysis and oxidative phosphorylation (by similarity suggests a role in adult tissue repair).

Q9H9Z2

Predicted band size: 23 kDa; Observed band size: 28 kDa

Affinity Chromatography

Non-conjugated

Unmodified

Neuroscience

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1/500-1/1000;IHC: 1/50-1/100;IF: 1/50-1/200;IP: 1/50;FC: 1/50-1/100

WB, IHC-P, ICC/IF, IP, FC

Liquid

Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human LIN28